Question

Which of the following dye is used for staining mitochondria?
A. Janus green
B. Safranin
C. Azure B
D. Crystal violet

Answer 1

Hint: The staining is a process where a particular chemical dye is used for a particular organism or organelles, and once it is stained, it looks different from the rest of the cellular components which are present.

Complete answer: Mitochondria is the powerhouse of the cell as they generate chemical energy which is needed for the cells to function. They are the membrane-bound organelles. The chemical energy produced is stored In the form of ATP in mitochondria. It has an outer as well as inner membrane. The outer membrane contains proteins such as porins. It plays an important role in the transport of ions. The Janus green is a basic dye which is also used as a vital stain in histology. This dye is used for staining the mitochondria. Janus Green changes the colour in accordance with the amount of the oxygen present, that is if oxygen is present the indicator is oxidized and the colour is turned blue and if the oxygen is absent, the indicator is reduced and the colour turns into pink.
Hence, the correct answer is option A.

Note: The safranin dye is used to stain the nuclei and the lignified walls of plant cells. The Azure B dye is a cationic dye that is used for staining the blood films. The crystal violets are used as a histological stain which is particularly used in gram staining for the classification of the bacteria.