Question

Which of the following statements are true?
a) Resolution power of the unaided human eye is 200μm.
b) Highest resolution is obtained with shortwave light.
c) Darkfield microscope is most useful for viewing living cells.
d) Chromatography is the method of separation of molecular components present in the cytosol.
e) In gel filtration chromatography, molecules can be separated in picogram to nanogram quantities.
A. a), b) and d) only.
B. a), c) and d) only.
C. b) and d) only.
D. c) and e) only.

Answer 1

Hint: The output of your eye is continuously integrated by our brain as it travels around in the picture you actually experience, and the effect is what you call Resolution. Chromatography is a mixture separation laboratory process. The light microscope, so named because it uses visible light to detect small objects, is perhaps the most well-known and well-used research tool in biology. The mixture is dissolved in a fluid. In the purification of carbohydrates, polysaccharides, nucleic acids, proteins, and other biological macromolecules, gel filtration plays a key role.

Complete answer:
Let us look at all the options to get the correct answer,
-The resolving capacity of the unaided human eye is 200μm under standard viewing conditions. A typical unaided human eye has a resolution of around 200 μm. By using a microscope, the resolution is reduced to distances as small as 0.2 μm. Resolution is the eye's property. The capacity of a lens to display two neighboring objects as distinct is resolving power.
-High resolution and sensitivity are provided by optical imaging using visible light. From 400 nm to 700 nm, the short-wave band.
-For observing living organisms, light microscopes are advantageous, but since individual cells are usually translucent, their components are not distinguishable unless they are colored with special stains.
-Chromatography is one of the most widely used methods of separating the molecular components left after centrifugation from the cells contained in a solution or cytosol.
-Gel filtration is a procedure in which component separation is based on the difference in the weight or size of the molecules. It is the simplest and mildest of all chromatography techniques and, based on variations in size, separates molecules.
Hence the correct answer is option A- a), b) and d) only.

Note: In the case of an optical device, the resolving power of a microscope is the most significant aspect. This is significant because it affects the ability to differentiate a specific specimen from fine details. When we speak about a microscope or telescope's resolving power, we mean its capacity to differentiate between two images when they are closely brought together.