Question

What is antisense technology?
A. When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene
B. RNA polymerase producing DNA
C. A cell displaying a foreign antigen used for synthesis of antigens
D. Production of somaclonal variants in tissue cultures

Answer 1

Hint: The non-coding DNA strand of a gene is antisense. An antisense DNA strand is used by a cell as a template to generate messenger RNA (mRNA) that guides a protein's synthesis.

Complete Answer:
- Antisense technology is where a fragment of RNA is used to interrupt the expression of a single gene that is complementary in the sequence.
- For genetic abnormalities or viruses, antisense therapy is a type of medication. If the genetic sequence of a single gene is understood to cause a certain disease, it is possible to synthesise a nucleic acid strand that attaches to and inactivates the messenger RNA formed by that gene, essentially shutting the gene off. This is because for it to be translated, mRNA needs to be single-stranded. Alternatively, the strand may be aimed to bind a pre-mRNA splicing site and alter an mRNA's exon material.

So the answer is “option A”.

Note: In different approaches, DNA / RNA physiology is applied. The most significant application used is Antisense Technology. Oligonucleotide is inserted into the cell here, which binds by complementary base-pairing to its target mRNA. This binding forms a cytoplasm RNA dimer which inhibits the synthesis of proteins.