Question

Explain the term Explant.

Answer 1

Hint: Any portion is taken from a plant or an animal that will be used to initiate a culture, where the portion might be a shoot or leaves even some other cells, the term explant can be applied to sample obtained from any part of the organism.

Complete answer:
- The process of how the explants are processed is extensively sterilized, and the culture can be used typically for 2 or 3 weeks.
- When coming to explants culture (in vivo) where it can be maintained in the laboratory for a short period.
- Whereas the in vivo type of explant culture allows investigators to perform experiments easily, and can also visualize the impact of tests.
- Ex vivo is another type of explant culture, where it requires a highly maintained environment and to recreate the original cellular condition.
- When coming to the procedure, the tissue is placed and harvested in the aseptic environment, whereas a sterile environment.
- The sample of the tissue, or explant often minced and placed in the cell culture that contains growth media.
- Progenitor cells migrate out of tissue on the surface of the plate, these cultured cells can be further expanded, transferred to the fresh dishes, through micropropagation.
- When coming to other applications of the explant and its culture is the field of biological research.
- In the case of organogenesis and morphogenesis of the fetus.
- As we know that explant cultured in the lab, the areas of the cells of interest can be labeled, with fluorescent markers.

Note: Explants culture can be labeled as transgenic labels that help to observe the growth of the specific cells, and this explant tissue and its culture can be used in the role of a specific gene, and also helpful in observing the gene expression.