Question

Match the types of WBC listed in column I with the shape of the nucleus given under column II. Choose the answer which gives the correct combination of alphabets of two columns.

Column I	Column II
	Type of WBC		Shape of Nucleus
a	Neutrophiles	p	Kidney shaped
b	Eosinophils	q	S-shaped
c	Basophils	r	3-5 lobes
d	Monocytes	s	2 lobes
		t	Disc-shaped

(a)a=r, b=t, c=p, d=q
(b)a=t, b=r, c=q, d=s
(c)a=q, b=p, c=t, d=r
(d)a=r, b=s, c=q, d=p

Answer 1

Hint: Different types of WBCs show different types of staining properties. The shape of the nucleus also varies in different leukocytes. The lobed nucleus is found in granulocytes. The number of lobes can vary from two to seven. In agranulocytes, lobes are not found but the nucleus shows unique shapes.

Complete answer:
-Mature neutrophils are purple in colour, multi- lobbed about two to six where lobes are connected with the chromatin filaments seen clearly through the cytoplasm.
-Eosinophils cells are not covered by the nucleus. Their nucleus is well defined but their lobs are not well defined.
-Basophils cells have a bilobed or trilobed 'S’ shaped nucleus.
-Monocytes contain a large eccentric nucleus of bean shape with fine chromatin.

Additional Information: -White blood cells are divided into two major groups: agranular and granular.
-Granular type of WBC contains primary granules- azurophilic and secondary granules- specific granules. It is further divided into neutrophils, eosinophil, and basophil.
- Agrnular type of WBC does not contain granules in their cytoplasm. It is further divided into monocytes and lymphocytes.
I-Neutrophils
-These cells have a horseshoe-shaped nucleus during the early stage but are further developed into a lobed nucleus.
-These cells take both acidic and basic stains and look violet-pink.
-The main function is phagocytosis, the reaction of inflammation and febrile response
II- Eosinophils
-These cells stain positive for peroxidase.
-They have granules like histamine, lysosomal enzyme, and ECF-A.
-They appear bright pink.
-The main function of these cells is phagocytosis, role in an allergic reaction, role in immunity, role in parasitic infection, etc.
III- Basophils
-They are irregular in shape as the boundary is not clearly defined because of overcrowding with coarse granules of heparin, histamine, and %- HT.
-These cells have a function of phagocytosis, role in an allergic reaction, the release of heparin, role in preventing the spread of allergic and inflammatory processes.
IV-Monocytes
-These cells resemble metamyelocytes.
-Monocytes have an eccentric or central round or oval nucleus.
-Monocytes from bone marrow migrate to the spleen

So, the correct answer is, ‘a=r, b=s, c=q, d=p.’

Note: -The process of development and maturation of WBC or leukocytes is called leukopoiesis, which is a part of hemopoiesis.
-Granulocytes and monocytes are divided from colony-forming units of granulocytes and monocytes.
-Leukocytes metabolism has aerobic glycolysis and active pentose phosphate pathway.