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The one located inside a vacuole is
A) Tonoplast
B) Matrix
C) Ergastic substances
D) Cell sap

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Answer
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Hint: Vacuole is a membrane bound organelle present in plant cell. They are closed compartments which do not have any fixed shape.

Complete Answer:
- Vacuoles are the storage organelles of the cell. They are larger in plant cells. Vacuoles are membrane bound organelles composed of phospholipids. It is simply a chamber which encloses water and other nutrients required by the cell known as cell sap. They are formed when multiple vesicles fuse together.
- Vacuoles act as the storage unit of the cell. They hold various substances and molecules. Vacuoles store food and other nutrients of the cell. It can even store waste products. They also store lipids, proteins and carbohydrates.
- Tonoplast – It is the membrane surrounding the vacuole. It separates the vacuole contents from the cytoplasm.
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Fig: Structure of Vacuole
 Ergastic substances are mainly present in the protoplasm. It contains cellulose and starch.
Matrix- It is present in the mitochondria. It contains mitochondrial DNA, small organic molecules, inorganic ions etc.

Thus the correct answer is (D) cell sap is present within vacuole.

Additional information: The function of the vacuoles depends on the cell in which it is present. For example; if it is present in fat cells, it will store lipids. Vacuoles show endocytosis and exocytosis function if present in an animal cell.

Note: A vacuole is a membranous compartment which contains water and other nutrients required for the cell to survive. A vacuole can retain its shape due to tonoplast.