Question

The source of the restriction enzyme HindIII is
A.Escherichia coli RY 13
B. Haemophilus influenzae Rd
C.Bacillus amyloliquefaciens H
D.Streptomyces albus

Answer 1

Hint: A protein that recognizes the specific nucleotide sequences and cuts the DNA only at that particular site is known as the restriction enzyme. A restriction enzyme is also called endonucleases, a protein produced by various bacteria.

Complete answer:
Escherichia coli RY 13: Also known as E.coli, it is a typical bacteria found in the human intestine as a regular part of the gut. E.coli strains can survive in an open environment, hence, making it suitable for environmental benefits.EcoRI is isolated from E.coli strain RY13. E.coli is the most significant contributor as a host in the gene cloning mechanism.

Haemophilus influenzae Rd: It is a gram-negative, facultatively anaerobic bacteria that belongs to the family Pasteurellaceae. HindIII is a type 2 specific site deoxyribonucleic restriction enzyme that is isolated from the Haemophilus influenzae Rd. It helps in the cleavage of DNA palindromic sequence in the presence of a cofactor.

Bacillus amyloliquefaciens H: It is a bacteria that is responsive to the BamHI restriction enzyme source. It is a gram-positive bacteria whose alpha-amylase is often used in starch hydrolysis. Bacillus amyloliquefaciens H is also a subtilisin source, which works similar to trypsin by catalyzing the breakdown of proteins.

Streptomyces albus: It has a minimized genome structure, so it is used for heterologous expression studies. Streptomyces is an antibiotic-producing genus that produces various types of antibacterial, antifungal, and antiparasitic drugs. They are of great use in the agricultural, medical, and veterinary industries.

So, the correct answer is "B- Haemophilus influenzae Rd."

Note:
Typically, HindIII has a complex structure and consists of a homodimer.HindIII is used in modern science, like DNA sequencing and mapping. They perform very specific cleaving of DNA. They are also used in gene cloning and molecular biology.