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The test-tube baby technique uses one of the following:
A. Intrauterine insemination (IUI)
B. Zygote intrafallopian transfer (ZIFT)
C. Micro sperm injection
D. Gamete intrafallopian transfer (GIFT)

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Last updated date: 27th Jun 2024
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Hint: Test-tube baby technique involves both in-vitro fertilization and then embryo transfer. The test-tube baby technique refers to a process of IVF or in vitro fertilization followed by embryo transfer. It is employed to treat infertility issues.

Complete answer: In this technique, the secondary oocyte and sperms are taken from the parents and fertilization takes place outside the female's body. After fertilization, the early embryo with more than 8 blastomeres can be transferred to the female body i.e. her fallopian tube or uterus.
Among the above-mentioned options, this can be done by a technique called ZIFT (Zygote Intrafallopian Transfer).
ZIFT is an assisted reproductive method quite similar to other such techniques.
The only difference is that the zygote is transferred into the fallopian tube and not into the uterus. Hence, it is also called tubal embryo transfer.

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Additional information: IUI or intrauterine insemination is a technique that involves placing of sperms into a woman's uterus to facilitate fertilization. Micro sperm injection is also known as intracytoplasmic sperm injection (ICSI).
In this process, a single sperm is chosen and then injected into the cytoplasm of the oocyte.
GIFT or gamete intra-fallopian transfer is a process that involves the transfer of sperm and eggs into the fallopian tubes for fertilization to occur.

So, the correct answer is B. Zygote Intrafallopian Transfer (ZIFT).

Note: ZIFT technique is used only when a blockage is present in a woman’s fallopian tube which prevents natural fertilization of sperm and ovum.