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Hint The tryptophan operon codes for the production of the tryptophan. The operon is autoregulated which means when the amount of tryptophan present is less the operon switches on and if the tryptophan level is more than the required amount then it is turned off.
Complete answer: In the trp operon there are five coding regions present which code for the production of the tryptophan. The coding region is the DNA sequence that has codes for the protein to decode. The promoter sequence present in the operon is located upstream of the transcriptional site. The operon has a sequence from which the binding of the protein can take place and the regulation of the operon can be done easily. The first coding region of the operon consists of the DNA code onto which the repressor protein can bind to it. When the tryptophan is present in the cells then the two tryptophan molecules bind to the trp repressor and it changes the shape to bind to the trp operator. This binding prevents the attachment of the RNA polymerase and then stops the transcription of the operon. When the tryptophan is not present in the cells the repressor is unable to bind itself to the operon. The operon is switched on and then the transcription of the tryptophan takes place. without the tryptophan present in the cell then the operon can’t be switched off. Tryptophan acts as a corepressor in this operon model.
So, the answer is ‘tryptophan and protein’.
Note: The trp operon is the negatively regulated operon as it operates when the can’t tryptophan level is lowered or is not present at all. When the amount of the tryptophan increases then the operon is switched off automatically.
Complete answer: In the trp operon there are five coding regions present which code for the production of the tryptophan. The coding region is the DNA sequence that has codes for the protein to decode. The promoter sequence present in the operon is located upstream of the transcriptional site. The operon has a sequence from which the binding of the protein can take place and the regulation of the operon can be done easily. The first coding region of the operon consists of the DNA code onto which the repressor protein can bind to it. When the tryptophan is present in the cells then the two tryptophan molecules bind to the trp repressor and it changes the shape to bind to the trp operator. This binding prevents the attachment of the RNA polymerase and then stops the transcription of the operon. When the tryptophan is not present in the cells the repressor is unable to bind itself to the operon. The operon is switched on and then the transcription of the tryptophan takes place. without the tryptophan present in the cell then the operon can’t be switched off. Tryptophan acts as a corepressor in this operon model.
So, the answer is ‘tryptophan and protein’.
Note: The trp operon is the negatively regulated operon as it operates when the can’t tryptophan level is lowered or is not present at all. When the amount of the tryptophan increases then the operon is switched off automatically.
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